期刊
JOURNAL OF REPRODUCTIVE IMMUNOLOGY
卷 121, 期 -, 页码 42-48出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.jri.2017.06.001
关键词
Pregnancy; Placenta; Vitamin D; Infections; Antimicrobials
资金
- National Institute of Food and Agriculture (NIFA)
- U.S. Department of Agriculture [2005-35200-15218, 2012-67017-30216]
- National Center for Research Resources (NCRR) [UL1 RR 024160]
- NIFA [578871, 2012-67017-30216] Funding Source: Federal RePORTER
Vitamin D is thought to modulate innate immune responses, and recent studies have highlighted the autocrine and paracrine functions of vitamin D in the placenta. Our objective was to determine the relationship between maternal vitamin D status and placental antimicrobial peptide (AMP) expression in a group of racially and ethnically diverse pregnant adolescents. In this study, 158 pregnant adolescents were recruited from the Rochester Adolescent Maternity Program (RAMP) in Rochester, NY. Maternal serum concentrations of the vitamin D biomarkers, 25-hydroxyvitamin D (25(OH)D) and 1,25-dihydroxyvitamin D (1,25(OH)(2)D), were measured at mid-gestation (similar to 26 weeks) and at delivery. At the placental level, vitamin D regulatory proteins (cubilin, megalin, la-hydroxylase (CYP27B1), 24-hydroxylase (CYP24A1), vitamin D receptor (VDR)) and AMPs (cathelicidin and hepcidin) were analyzed using quantitative PCR and western blot techniques. Placental CYP27B1 mRNA expression was significantly positively associated with both placental cathelicidin mRNA expression (P < 0.0001) and placental hepcidin mRNA expression (P = 0.002). In teens with positive recto -vaginal group B streptococcus (GBS) colonization, placental mRNA expression of cathelicidin (P = 0.007), cubilin (P = 0.03), and CYP27B1 (P = 0.04) were significantly lower compared to those who tested negative for this infection. A mediation analysis showed that the indirect relationship between GBS colonization and placental cathelicidin mRNA expression was mediated by the placental mRNA expression of the vitamin D proteins cubilin and CYP27B1 (P = 0.02). Additional research is needed to identify the role and relative contributions of placental and systemic vitamin D metabolites in relation to potentially pathogenic microorganisms which may be present during pregnancy.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据