期刊
ANALYST
卷 144, 期 18, 页码 5413-5419出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c9an01042d
关键词
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资金
- Global Experts Recruitment Program of China
- Innovative and Entrepreneurial Talent Recruitment Program of Jiangsu Province
- State Key Project of Research and Development [2016YFF0100802]
- Fundamental Research Funds for the Central Universities [2242018K41023]
- Science and Technology Development Project of Nanjing [201716006]
- Key Project and Open Research Fund of State Key Laboratory of Bioelectronics, Southeast University
Herein, a chip imitating the desert beetle shell was presented for naked eye nucleic acid quantification. The hydrophobic photonic crystal substrate treated by ultraviolet local irradiation could effectively disperse the sample into hundreds of droplets for digital loop-mediated isothermal amplification (dLAMP). Pyrophosphate (PPI), a by-product of the LAMP reaction, combined with magnesium ions to form a poorly soluble precipitate. It could be fixed on a silica substrate due to complexation, resulting in the disappearance of the structural color of the photonic crystals. The number of points without structural color contains the information of the copy number of nucleic acids in the sample. This chip could achieve the naked eye quantitative detection of Salmonella DNA without fluorescence or other chromogenic reagents. Thus, the chip designed in this study can help the development of digital nucleic acid detection under limited resource settings (LRS) and can be suitable for POCT (point of care test) standards.
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