期刊
JOURNAL OF PROTEOME RESEARCH
卷 16, 期 2, 页码 842-851出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jproteome.6b00824
关键词
single amino acid variant; breast cancer; MCF-7 cell line; cell line subpopulation; proteomics
资金
- National Institutes of Health [R01 GM49500]
- NIH Center Grant [P30 ES 020957, P30 CA 022453]
- NIH Shared Instrumentation Grant [S10 OD 010700]
Cancers are initiated and developed from a small population of stem-like cells termed cancer stem cells (CSCs). There is heterogeneity among this CSC population that leads to multiple subpopulations with their own distinct biological features and protein expression. The protein expression and function may be impacted by amino acid variants that can occur largely due to single nucleotide changes. We have thus performed proteomic analysis of breast CSC subpopulations by mass spectrometry to study the presence of single amino acid variants (SAAVs) and their relation to breast cancer. We have used CSC markers to isolate pure breast CSC subpopulation fractions (ALDH+ and CD44+/CD24- cell populations) and the mature luminal cells (CD49f-EpCAM+) from the MCF-7 breast cancer cell line. By searching the Swiss-CanSAAVs database, 374 unique SAAVs were identified in total, where 27 are cancer-related SAAVs. 135 unique SAAVs were found in the CSC population compared with the mature luminal cells. The distribution of SAAVs detected in MCF-7 cells was compared with those predicted from the Swiss-CanSAAVs database, where we found distinct differences in the numbers of SAAVs detected relative to that expected from the Swiss-CanSAAVs database for several of the amino acids.
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