期刊
JOURNAL OF PROTEOME RESEARCH
卷 16, 期 11, 页码 4113-4121出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jproteome.7b00486
关键词
human milk; alpha-casein; beta-casein; kappa-casein; total protein; whey/casein ratio; label-free LC-MS/MS; amino acid analysis; iBAQ
资金
- Australian Research Council (ARC) Development Early Career Research Awards (DECRA) [DE160100382]
- Mead Johnson Nutrition
Whey proteins and caseins in breast milk provide bioactivities and also have different amino acid composition. Accurate determination of these two major protein classes provides a better understanding of human milk composition and function, and further aids in developing improved infant formulas based on bovine whey proteins and caseins. In this study, we implemented a LC-MS/MS quantitative analysis based on iBAQ label-free quantitation, to estimate absolute concentrations of alpha-casein, beta-casein, and kappa-casein in human milk samples (n = 88) collected between day 1 and day 360 postpartum. Total protein concentration ranged from 2.03 to 17.52 with a mean of 9.37 +/- 3.65 g/L. Casein subunits ranged from 0.04 to 1.68 g/L (alpha-), 0.04 to 4.42 g/L (beta-), and 0.10 to 1.72 g/L (alpha-), with beta-casein having the highest average concentration among the three subunits. Calculated whey/casein ratio ranged from 45:55 to 97:3. Linear regression analyses show significant decreases in total protein, beta-casein, kappa-casein, total casein, and a significant increase of whey/casein ratio during the course of lactation. Our study presents a novel and accurate quantitative analysis of human milk casein content, demonstrating a lower casein content than earlier believed, which has implications for improved infants formulas.
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