期刊
JOURNAL OF PROTEOME RESEARCH
卷 16, 期 7, 页码 2597-2613出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jproteome.7b00163
关键词
Streptomyces; glycolytic metabolism; oxidative metabolism; oxidative stress; dormancy regulon; secondary metabolites; tellurium resistance proteins; shotgun label-free quantitative proteomics; mass spectrometry
资金
- CNRS
- Universite Paris Sud
- ANR Bio-SoundIR of the French National Research Agency [ANR-1S-CE09-000201]
- National Science and Technology Council (CONACYT, Mexico) [314457]
Recent physiological studies indicated that S. lividans metabolism was mainly glycolytic, whereas S. coelicolor Metabolism was mainly oxidative. To determine whether such metabolic characteristics were correlated with consistent proteomics features, a comparative label-free, shotgun proteomics analysis of these strains was carried out. Among 2024 proteins identified; 360 showed significant differences in abundance between the strains. This study revealed that S. coelicolor catabolized glucose less actively than S. lividans, whereas the amino acids present in the medium were catabolized less actively by S. lividans than by S. coelicolor. The abundance of glycolytic proteins in S. lividans was consistent with its high glycolytic activity, whereas the abundance of proteins involved in the catabolism of amino acids in S. coelicolor provided an explanatory basis for its predominantly oxidative metabolism. In this study, conducted under conditions of low O-2 availability, proteins involved in resistance to oxidative stress and those belonging to a DosR-like dormancy regulon were abundant in S. coelicolor, whereas tellurium resistance proteins were abundant in S. lividans. This indicated that the strains reacted differently to O-2 limitation. Proteins belonging to the CDA, RED, and ACT pathways, usually highly expressed in S. coelicolor, were not detected under these Conditions, whereas proteins of siderophores, 5-hydroxyectoine, and terpenoid biosynthetic pathways were present.
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