期刊
JOURNAL OF PROTEOME RESEARCH
卷 16, 期 12, 页码 4330-4339出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jproteome.7b00353
关键词
missing proteins; ProteoMiner protein enrichment kit; human tissue; LC-MS/MS; Triton X-100
资金
- National Basic Research Program of China [2014CBA02002, 2014CBA02005]
- National Natural Science Foundation of China [31500670]
Human Proteome Project (HPP) aims at mapping entire human proteins with a systematic effort upon all the emerging techniques, which would enhance understanding of human biology and lay a foundation for development of medical applications. Until now, 2563 missing proteins (MPs, PE2-4) are still undetected even using the most sensitive approach of protein detection. Herein, we propose that enrichment of low-abundance proteins benefits MPs finding. ProteoMiner is an equalizing technique by reducing high-abundance proteins and enriching low abundance proteins in biological liquids. With triton X-100/TBS buffer extraction, ProteoMiner enrichment, and peptide fractionation, 20 MPs (at least two non-nested unique peptides with more than eight a.a. length) with 60 unique peptides were identified from four human tissues induding eight membrane/secreted proteins five nucleus proteins. Then confirmed with two non-nested unique peptides (>= 9 a.a.) identified by matching well with their chemically synthetic peptides in PRM assay. Hence, these results demonstrated ProteoMiner as a powerful means in discovery of MPs.
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