期刊
JOURNAL OF PROTEOME RESEARCH
卷 16, 期 11, 页码 4020-4034出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jproteome.7b00412
关键词
silver nanoparticles NM-300; protein corona; O-18 quantitative mass spectrometry; Caco-2 cells; proteomics; transcriptomics
资金
- German Research Foundation/Deutsche Forschungsgemeinschaft (DFG) [LA 1177/9-1]
- German Federal Institute for Risk Assessment [1322-554, 1332-629]
The breadth of applications of nanoparticles and the access to food-associated consumer products containing nanosized materials lead to oral human exposure to such particles. In biological fluids nanoparticles dynamically interact with biomolecules and form a protein corona. Knowledge about the protein corona is of great interest for understanding the molecular effects of particles as well as their fate inside the human body. We used a mass spectrometry-based toxicoproteomics approach to elucidate mechanisms of toxicity of silver nanoparticles and to comprehensively characterize the protein corona formed around silver nanoparticles in Caco-2 human intestinal epithelial cells. Results were compared with respect to the cellular function of proteins either affected by exposure to nanoparticles or present in the protein corona. A transcriptomic data set was included in the analyses in order to obtain a combined multiomics view of nanoparticle-affected cellular processes. A relationship between corona proteins and the proteomic or transcriptomic responses was revealed, showing that differentially regulated vroteins or transcripts were engaged in the same cellular signaling pathways. Protein corona analyses of nanoparticles in cells might therefore help in obtaining information about the molecular consequences of nanoparticle treatment.
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