期刊
HELIYON
卷 5, 期 8, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.heliyon.2019.e02234
关键词
Food analysis; Food composition; Food chemistry; Immunology; Pharmaceutical science; Response surface method; Antioxidant; Analgesic; Tribulus terrestris L. leaves; Anti-inflammatory; Antibacterial; Flavonoids; Composition analysis
资金
- Natural Science Foundation of Liaoning Province of China [20180550647]
- Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University), Ministry of Education
Research on the extraction technology, component analysis, and antioxidant, antibacterial, analgesic and anti-inflammatory activities of flavonoids fraction from Tribulus terrestris L. leaves was carried out. The extraction process was optimized by response surface method, and the maximum yield 0.27% for flavonoids fraction was achieved with concentration of ethanol solution 25.87%, ratio of solvent to material 30:1 ml/g, and ultrasonic extraction time 27.93 min. Moreover, 14 compositions were identified separately from flavonoids fraction by HPLC-DAD-ESI-MS2. In addition, flavonoids fraction exhibited a better antioxidant activity demonstrated by assays of ABTS, DPPH radical scavenging activity and ferric reducing antioxidant power activity. Furthermore, the antibacterial, analgesic and anti-inflammatory activities of flavonoids fraction were also proved to be stronger. Therefore, the present findings suggest that flavonoids fraction from Tribulus terrestris L. leaves can be a very interesting candidate for the research and development of natural and healthy herbal medicine for the pharmaceutical and food industries.
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