期刊
JOURNAL OF PLANT RESEARCH
卷 130, 期 3, 页码 587-598出版社
SPRINGER JAPAN KK
DOI: 10.1007/s10265-017-0924-6
关键词
Casein kinase II; ABA; GA; Signaling; T-DNA mutants; Over-expression
资金
- National Key Laboratory of Plant Molecular Genetics
- National Natural Science Foundation of China [31540064, 31071076, 30871325]
- Program for New Century Excellent Talents in University [NCET-10-0363]
- Excellent Youth Foundation of Hunan Province [11JJ1005]
- Ph.D. Programs Foundation of Ministry of Education of China [20130161110005]
- Hunan Provincial Innovation Foundation for Postgraduate [CX2015B073, CX2016B097]
- Key Research and Development Project of Hunan Provincial Department of Science and Technology [2016WK2003]
Casein kinase II (CK2), an evolutionarily well-conserved Ser/Thr kinase, plays critical roles in all higher organisms including plants. CKB1 is a regulatory subunit beta of CK2. In this study, homozygous T-DNA mutants (ckb1-1 and ckb1-2) and over-expression plants (35S:CKB1-1, 35S:CKB1-2) of Arabidopsis thaliana were studied to understand the role of CKB1 in abiotic stress and gibberellic acid (GA) signaling. Histochemical staining showed that although CKB1 was expressed in all organs, it had a relatively higher expression in conducting tissues. The ckb1 mutants showed reduced sensitivity to abscisic acid (ABA) during seed germination and seedling growth. The increased stomatal aperture, leaf water loss and proline accumulation were observed in ckb1 mutants. In contrast, the ckb1 mutant had increased sensitivity to polyaluminum chloride during seed germination and hypocotyl elongation. We obtained opposite results in over-expression plants. The expression levels of a number of genes in the ABA and GA regulatory network had changed. This study demonstrates that CKB1 is an ABA signaling-related gene, which subsequently influences GA metabolism, and may play a positive role in ABA signaling.
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