期刊
JOURNAL OF PLANT PHYSIOLOGY
卷 215, 期 -, 页码 20-29出版社
ELSEVIER GMBH
DOI: 10.1016/j.jplph.2017.04.015
关键词
Rice oleosin; Promoter analysis; Cis-regulatory element; Abscisic acid; Suspension cultured cell
资金
- Cooperative Research Program for Agriculture Science and Technology Development, Rural Development Administration [PJ01194202]
- Basic Core Technology Development Program for the Oceans and the Polar Regions of the National Research Foundation (NRF) - Ministry of Science, ICT&Future Planning, Republic of Korea [NRF-2015M1A5A1037053]
Oleosins are the most abundant proteins in the monolipid layer surrounding neutral storage lipids that form oil bodies in plants. Several lines of evidence indicate that they are physiologically important for the maintenance of oil body structure and for mobilization of the lipids stored inside. Rice has six oleosin genes in its genome, the expression of all of which was found to be responsive to abscisic acid (ABA) in our examination of mature embryo and aleurone tissues. The 5'-flanking region of OsOle5 was initially characterized for its responsiveness to ABA through a transient expression assay system using the protoplasts from suspension-cultured rice cells. A series of successive deletions and site-directed mutations identified five regions critical for the hormonal induction of its promoter activity. A search for cis-acting elements in these regions deposited in a public database revealed that they contain various promoter elements previously reported to be involved in the ABA response of various genes. A gain-of-function experiment indicated that multiple copies of all five regions were sufficient to provide the minimal promoter with a distinct ABA responsiveness. Comparative sequence analysis of the short, but still ABA-responsive, promoters of OsOle genes revealed no common modular architecture shared by them, indicating that various distinct promoter elements and independent trans-acting factors are involved in the ABA responsiveness of rice oleosin multigenes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据