期刊
JOURNAL OF PLANT PHYSIOLOGY
卷 208, 期 -, 页码 7-16出版社
ELSEVIER GMBH
DOI: 10.1016/j.jplph.2016.10.011
关键词
Thlaspi arvense; Erucic acid; Seed oil; TAG (triacylglycerol); FAE1 (fatty acid elongase 1); Biofuel
资金
- Spanish Ministry of Economy and Competitiveness [MINECO AGL2014-55300-R]
- Aragon Government [E33]
We studied erucic acid accumulation in the biodiesel feedstock Pennycress (Thlaspi arvense L.) as a first step towards the development of a sustainable strategy for biofuel production in the EU territory. To that end, two inbred Pennycress lines of European origin, NASC and French, were cultivated in a controlled chamber and in experimental field plots, and their growth, seed production and seed oil characteristics analyzed. Differences in some agronomical traits like vernalization (winter-French versus spring-NASC), flowering time (delayed in the French line) and seed production (higher in the French line) were detected. Both lines showed a high amount (35-39%)of erucic acid (22:1(boolean AND 13)) in their seed oil. Biochemical characterization of the Pennycress seed oil indicated that TAG was the major reservoir of 22:1(Delta 13). Incorporation of 22:1(Delta 13) to TAG occurred very early during seed maturation, concomitant with a decrease of desaturase activity. This change in the acyl fluxes towards elongation was controlled by different genes at different levels. TaFAE1 gene, encoding the fatty acid elongase, seemed to be controlled at the transcriptional level with high expression at the early stages of seed development. On the contrary, the TaFAD2 gene that encodes the Delta 12 fatty acid desaturase or TaDGAT1 that catalyzes TAG biosynthesis were controlled post-transcriptionally. TaWRI1, the master regulator of seed-oil biosynthesis, showed also high expression at the early stages of seed development. Our data identified genes and processes that might improve the biotechnological manipulation of Pennycress seeds for high-quality biodiesel production. (C) 2016 Elsevier GmbH. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据