期刊
JOURNAL OF PLANT BIOLOGY
卷 60, 期 3, 页码 259-267出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s12374-016-0905-7
关键词
Arachis hypogaea L.; K+/H+ Antiporter; K+ transport; K+ uptake; Salt stress
资金
- National Natural Science Foundation of China [31571581, 31571605]
- Natural Science Foundation of Shandong Province [ZR2015YL077]
- Project of Significant Application of Agricultural Technology Innovation in Shandong Province, International Science & Technology Cooperation Program of China [2015DFA31190]
- Supporting Plan of National Science and Technology of China [2014BAD11B04]
- earmarked fund for Modern Agroindustry Technology Research System [CARS14]
- Shandong Major Projects of Independent Innovation Achievement Transformation [2012ZHZXIA0418]
High salinity is the one of important factors limiting plant growth and crop production. Many NHX-type antiporters have been reported to catalyze K+/H+ exchange to mediate salt stress. This study shows that an NHX gene from Arachis hypogaea L. has an important role in K+ uptake and transport, which affects K+ accumulation and plant salt tolerance. When overexpressing AhNHX1, the growth of tobacco seedlings is improved with longer roots and a higher fresh weight than the wild type (WT) under NaCl treatment. Meanwhile, when exposed to NaCl stress, the transgenic seedlings had higher K+/H+ antiporter activity and their roots got more K+ uptake. NaCl stress could induce higher K+ accumulation in the roots, stems, and leaves of transgenic tobacco seedlings but not Na+ accumulation, thus, leading to a higher K+/Na+ ratio in the transgenic seedlings. Additionally, the AKT1, HAK1, SKOR, and KEA genes, which are involved in K+ uptake or transport, were induced by NaCl stress and kept higher expression levels in transgenic seedlings than in WT seedlings. The H+-ATPase and H+-PPase activities were also higher in transgenic seedlings than in the WT seedlings under NaCl stress. Simultaneously, overexpression of AhNHX1 increased the relative distribution of K+ in the aerial parts of the seedlings under NaCl stress. These results showed that AhNHX1 catalyzed the K+/H+ antiporter and enhanced tobacco tolerance to salt stress by increasing K+ uptake and transport.
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