期刊
NATURE PLANTS
卷 5, 期 10, 页码 1043-1056出版社
NATURE PORTFOLIO
DOI: 10.1038/s41477-019-0509-6
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资金
- National Science Foundation Plant-Biotic Interactions Program [1758976]
- Department of Energy Joint Genome Institute Community Science Program [CSP2568]
- National Science Foundation Graduate Research Fellowship Program
- Division Of Integrative Organismal Systems
- Direct For Biological Sciences [1758976] Funding Source: National Science Foundation
Duplication and divergence of primary pathway genes underlie the evolution of plant specialized metabolism; however, mechanisms partitioning parallel hormone and defence pathways are often speculative. For example, the primary pathway intermediate ent-kaurene is essential for gibberellin biosynthesis and is also a proposed precursor for maize antibiotics. By integrating transcriptional coregulation patterns, genome-wide association studies, combinatorial enzyme assays, proteomics and targeted mutant analyses, we show that maize kauralexin biosynthesis proceeds via the positional isomer ent-isokaurene formed by a diterpene synthase pair recruited from gibberellin metabolism. The oxygenation and subsequent desaturation of ent-isokaurene by three promiscuous cytochrome P45Os and a new steroid 5 alpha reductase indirectly yields predominant ent-kaurene-associated antibiotics required for Fusarium stalk rot resistance. The divergence and differential expression of pathway branches derived from multiple duplicated hormone-metabolic genes minimizes dysregulation of primary metabolism via the circuitous biosynthesis of ent-kaurene-related antibiotics without the production of growth hormone precursors during defence.
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