4.8 Article

Label-Free Vibrational Spectroscopic Imaging of Neuronal Membrane Potential

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JOURNAL OF PHYSICAL CHEMISTRY LETTERS
卷 8, 期 9, 页码 1932-1936

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AMER CHEMICAL SOC
DOI: 10.1021/acs.jpclett.7b00575

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  1. Keck Foundation
  2. Brain and Behavior Research Foundation
  3. NIH [T32 GM077229]
  4. [R00DA030396]

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Detecting membrane potentials is critical for understanding how neuronal networks process information. We report a vibrational spectroscopic signature of neuronal membrane potentials identified through hyperspectral stimulated Raman scattering (SRS) imaging of patched primary neurons. High-speed SRS imaging allowed direct visualization of puff-induced depolarization of multiple neurons in mouse brain slices, confirmed by simultaneous calcium imaging. The observed signature, partially dependent on sodium ion influx, is interpreted as ion interactions on the CH3 Fermi resonance peak in proteins. By implementing a dual-SRS balanced detection scheme, we detected single action potentials in electrically stimulated neurons. These results collectively demonstrate the potential of sensing neuronal activities at multiple sites with a label-free vibrational microscope.

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