期刊
PAN AFRICAN MEDICAL JOURNAL
卷 33, 期 -, 页码 -出版社
AFRICAN FIELD EPIDEMIOLOGY NETWORK-AFENET
DOI: 10.11604/pamj.2019.33.285.18776
关键词
Nepeta crispa; cytotoxicity; essential oil; invertebrate cell line; malathion; vertebrate cell line
资金
- Hamadan University of Medical Sciences [9605033004]
Introduction: pesticides are used as essential tools to control vector-borne diseases and agricultural pests and maintain quality and quantity crop production. Scientists attempt to use derived plant natural products due to environmental safety and low mammalian toxicity. Therefore, the cytotoxicity of malathion and Nepeta crispa essential oil against vertebrate L929 and invertebrate Sf9 cell lines were investigated. Methods: about 2x10(3) cells were placed into the wells of a 96-well plate experiments. Then appropriate concentrations of malathion and N. crispa essential oil added to the wells. The cells were allowed to grow for 3-5 days and estimated the cell numbers. Control cell wells contained only cells with DMSO. All treatments and controls repeated at least four replicates. Results: about 2x10(3) cells were placed into the wells of a 96-well plate experiments. Then appropriate concentrations of malathion and N. crispa essential oil added to the wells. The cells were allowed to grow for 3-5 days and estimated the cell numbers. Control cell wells contained only cells with DMSO. All treatments and controls repeated at least four replicates. Conclusion: plant essential oil not only had no negative effects but also had boosting effects on the L929 cell viability. Nepeta crispa essential oil had negative effects on the Sf9 cell viability with the differences that derived plant natural products containing environmentally friendly and readily biodegradable derivatives, hydrolyzing rapidly in nature and nearly having no destructive effects on mammals and environment.
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