期刊
JOURNAL OF PHARMACEUTICAL SCIENCES
卷 106, 期 9, 页码 2302-2311出版社
WILEY
DOI: 10.1016/j.xphs.2017.05.010
关键词
biliary excretion; hepatocytes; ADME; hepatobiliary disposition; cell culture; tissue engineering; toxicology; membrane transport/transporters; disposition; metabolism
Tissue engineering technology has provided many useful culture models. This article reviews the merits of this technology in a hepatocyte culture system and describes the applications of the sandwich-cultured hepatocyte model in drug discovery. In addition, we also review recent investigations of the utility of the 3-dimensional bioprinted human liver tissue model and spheroid model. Finally, we present the future direction and developmental challenges of a hepatocyte culture model for the successful establishment of a microphysiological system, represented as an organ-on-a-chip and even as a human-on-a-chip. A merit of advanced culture models is their potential use for detecting hepatotoxicity through repeated exposure to chemicals as they allow long-term culture while maintaining hepatocyte functionality. As a future direction, such advanced hepatocyte culture systems can be connected to other tissue models for evaluating tissue-to-tissue interaction beyond cell-to-cell interaction. This combination of culture models could represent parts of the human body in a microphysiological system. (C) 2017 American Pharmacists Association (R). Published by Elsevier Inc. All rights reserved.
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