Simultaneous trace monitoring of prokinetic drugs in human plasma using magnetic dispersive micro-solid phase extraction based on a new graphene oxide/metal-organic framework-74/Fe3O4/polytyramine nanoporous composite in combination with HPLC

In this work, graphene oxide/metal-organic framework-74/Fe3O4/polytyramine (GO/MOF-74/Fe3O4/PTy) nanoporous composite was synthesized as a sustainable sorbent for magnetic dispersive micro-solid phase extraction (MD-mu-SPE) of prokinetic drugs. The new nanosacle sorbent in combination with high performance liquid chromatography-ultraviolent detection (HPLC-UV) was applied for simultaneous trace quantification of target drugs in human plasma. MOF-74 and PTy were used as surface modifiers to improve the properties of GO nanosheets, such as surface area-to-volume ratio, adsorption capacity, hydrophobic interactions and selectivity. A composite of GO/MOF-74/Fe3O4 was fabricated and then an oxidative polymerization of tyramine was performed on the surface of sorbent using horseradish peroxidaze (HRP) enzyme as a catalyst. The characterization of the hybrid sorbent was evaluated using scanning electron microscopy (SEM), energy-dispersive X-ray analysis (EDX), X-ray diffraction (XRD) and Fourier transform-infrared (FT-IR) spectroscopy. The limit of detections (LODs, S/N = 3) for domperidone (DOM) and itopride (ITP) were 0.4 and 1.1 ng mL(-1), respectively. Notable linearity (0.995 >= r(2) >= 0.991) and practical dynamic concentration ranges of 1.5-1100.0 ng mL(-1) and 4.0-1750.0 ng mL(-1) were obtained for DOM and ITP, respectively. Intra-assay (<= 8.6%, n = 12) and inter-assay (<= 9.0%, n = 12) precisions along with appreciable accuracies (<= 9.3%) demonstrated satisfactory performance of the current method. Ultimately, this approach was utilized for trace monitoring of DOM and ITP in human plasma after low dose administration and some pharmacokinetic features were investigated in detail.