4.5 Article

Cimetidine Reduces Interleukin-6, Matrix Metalloproteinases-1 and-9 Immunoexpression in the Gingival Mucosa of Rat Molars With Induced Periodontal Disease

期刊

JOURNAL OF PERIODONTOLOGY
卷 88, 期 1, 页码 100-111

出版社

WILEY
DOI: 10.1902/jop.2016.160132

关键词

Cimetidine; gingiva; immunohistochemistry; interleukin-6; osteoclast; periodontitis

资金

  1. Sao Paulo Research Foundation [2010/10391-9, 2010/06912-3]
  2. National Council for Scientific and Technological Development, Brazil [309533/2015-5]
  3. Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [10/10391-9, 10/06912-3] Funding Source: FAPESP

向作者/读者索取更多资源

Background: Histamine seems to act, via H-2 receptor, on inflammatory processes by stimulating interleukin (IL)-6 and matrix metalloproteinase (MMP) release. As cimetidine is an H-2 receptor antagonist, the authors hypothesize that this antiulcer drug reduces IL-6, MMP-1, andMMP-9immunoexpressioningingiva with inducedperiodontal disease (PD). Toconfirmapossible modulatory role of IL-6 on MMPs, the relationship between IL-6/MMP-1 and IL-6/MMP-9 immunoexpression was evaluated. Methods: Forty-six male rats were distributed into the cimetidine group (CimG: received daily intraperitoneal injections of 100 mg/kg of body weight of cimetidine) or the saline group (SG). PD was induced by cotton ligature around the maxillary left first molars (PDSG and PDCimG). The right molars were used as controls (SG and CimG). After 7, 15, 30, and 50 days, maxillary fragments were processed for paraffin embedding or for transmission electron microscopy. Tartrateresistant acid phosphatase (TRAP)-positive osteoclasts in the alveolar process surface and number of IL-6, MMP-1, and MMP-9-immunolabeled cells in the gingival mucosa were quantified. Statistical analyses were performed (P <= 0.05). Results: In PDSG and PDCimG, gingival mucosa exhibited few collagen fibers among numerous inflammatory cells. In PDCimG, the number of TRAP-positive osteoclasts and IL-6, MMP-1, andMMP-9-immunolabeled cells was significantly lower than in PDSG at all periods. A positive correlation between IL-6/MMP-1 and IL-6/MMP-9 was detected in PDSG and PDCimG. Conclusion: Cimetidine decreases bone loss through reduction of osteoclast number and induces reduction of IL-6, MMP-1, and MMP-9 immunoexpression, reinforcing the idea that the beneficial effect of cimetidine in PD may be due to reduction of IL-6 immunolabeling in the inflamed gingival mucosa.

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