期刊
JOURNAL OF PATHOLOGY
卷 244, 期 1, 页码 84-96出版社
WILEY
DOI: 10.1002/path.4993
关键词
lipocalin-type prostaglandin D synthase; endothelial cell; tumor vessel; angiogenesis; vascular permeability; endothelial-to-mesenchymal transition; melanoma; lung carcinoma; oral squamous cell carcinoma
资金
- Japan Society for the Promotion of Science [25252049, 26660225]
- Ono Medical Research Foundation
- Cardiovascular Research Fund
- Kurozumi Medical Foundation
- Naito Foundation
- Suzuken Memorial Foundation
- Takeda Science Foundation
- Nipponham Foundation
- Sapporo Bioscience Foundation
- Japan Foundation for Pediatric Research
- [10795]
- Grants-in-Aid for Scientific Research [25252049, 26660225, 17K15611] Funding Source: KAKEN
Endothelial cells (ECs) are a key component of the tumor microenvironment. They have abnormal characteristics compared to the ECs in normal tissues. Here, we found a marked increase in lipocalin-type prostaglandin D synthase (L-PGDS) mRNA (Ptgds) expression in ECs isolated from mouse melanoma. Immunostaining of mouse melanoma revealed expression of L-PGDS protein in the ECs. In situ hybridization also showed L-PGDS (PTGDS) mRNA expression in the ECs of human melanoma and oral squamous cell carcinoma. In vitro experiments showed that stimulation with tumor cell-derived IL-1 and TNF-alpha increased L-PGDS mRNA expression and its product prostaglandin D-2 (PGD(2)) in human normal ECs. We also investigated the contribution of L-PGDS-PGD(2) to tumor growth and vascularization. Systemic or EC-specific deficiency of L-PGDS accelerated the growth of melanoma in mice, whereas treatment with an agonist of the PGD(2) receptor, DP1 (BW245C, 0.1mg/kg, injected intraperitoneally twice daily), attenuated it. Morphological and in vivo studies showed that endothelial L-PGDS deficiency resulted in functional changes of tumor ECs such as accelerated vascular hyperpermeability, angiogenesis, and endothelial-to-mesenchymal transition (EndMT) in tumors, which in turn reduced tumor cell apoptosis. These observations suggest that tumor cell-derived inflammatory cytokines increase L-PGDS expression and subsequent PGD(2) production in the tumor ECs. This PGD(2) acts as a negative regulator of the tumorigenic changes in tumor ECs. Copyright (C) 2017 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
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