期刊
FOOD AND AGRICULTURAL IMMUNOLOGY
卷 30, 期 1, 页码 1162-1173出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09540105.2019.1674787
关键词
Cimaterol; indirect competitive enzyme-linked immunosorbent assay (ic-ELISA); immunochromatographic test strip; urine
资金
- National Key RD Program [2018YFC1602900]
An anti-cimaterol (cima) monoclonal antibody, 2H8, was prepared and used to develop an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and an immunochromatographic test strip assay for the detection of cima, a newly described ?-agonist. The half-maximal inhibitory concentration of the antibody was 1.56?ng/mL, and no cross-reactivity with other ?-adrenergic agonists was observed. The antibody?s limit of detection was 0.416?ng/mL, and its linear range of detection was 0.416?5.868?ng/mL. The recovery rates of cima in swine urine ranged from 86% to 92.5%, and the test strip had a cut-off value of 10?ng/mL. Both ic-ELISA and the developed strip assay are effective when detecting cima in swine urine.
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