期刊
JOURNAL OF ORAL PATHOLOGY & MEDICINE
卷 47, 期 1, 页码 91-95出版社
WILEY
DOI: 10.1111/jop.12645
关键词
epigenetic; inflammation; lichen planus; methylation; oral lichen planus
资金
- Coordination for the Improvement of Higher Education Personnel
- National Council for Scientific and Technological Development
- Foundation of the State of Minas Gerais (FAPEMIG)/Brazil
- Pro-Reitoria de Pesquisa of the Universidade Federal de Minas Gerais/Brazil
BackgroundThe oral lichen planus is a chronic inflammatory disease. Although its aetiology is not well understood, the role of T lymphocytes in its inflammatory events is recognised. Identifying the epigenetic mechanisms involved in the pathogenesis of this immune-mediated condition is fundamental for understanding the inflammatory reaction that occurs in the disease. The purpose of this work was to evaluate the methylation pattern of 21 immune response-related genes in the different clinical forms of oral lichen planus. MethodsA cross-sectional study was performed to analyse the DNA methylation patterns in three distinct groups of oral lichen planus: (i) reticular/plaque lesions; (ii) erosive lesions; (iii) normal oral mucosa (control group). After DNA extraction from biopsies, the samples were submitted to digestions by methylation-sensitive and methylation-dependent enzymes and double digestion. The relative percentage of methylated DNA for each gene was provided using real-time polymerase chain reaction arrays. ResultsHypermethylation of the STAT5A gene was observed only in the control group (59.0%). A higher hypermethylation of the ELANE gene was found in reticular/plaque lesions (72.1%) compared to the erosive lesions (50.0%). ConclusionOur results show variations in the methylation profile of immune response-related genes, according to the clinical type of oral lichen planus after comparing with the normal oral mucosa. Further studies are necessary to validate these findings using gene expression analysis.
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