期刊
GLYCOBIOLOGY
卷 29, 期 11, 页码 750-754出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/glycob/cwz058
关键词
enzymatic labeling; fluorescent labeling; glycan labeling; sialic acid; sialyltransferase
资金
- Bio-Techne
Glycosylation is a common modification found on numerous proteins and lipids. However, direct detection of glycans on these intact biomolecules has been challenge. Here, utilizing enzymatic incorporation of fluorophore-conjugated sialic acids, dubbed as direct fluorescent glycan labeling, we report the labeling and detection of N- and O-glycans on glycoproteins. The method allows detection of specific glycans without the laborious gel blotting and chemiluminescence reactions used in Western blotting. The method can also be used with a variety of fluorescent dyes.
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