期刊
FEMS MICROBIOLOGY LETTERS
卷 366, 期 10, 页码 -出版社
OXFORD UNIV PRESS
DOI: 10.1093/femsle/fnz108
关键词
Ustilago maydis; vacuole; autophagosomes; proteinase A; Pep4p; PEP4
类别
资金
- Consejo Nacional de Ciencia y Tecnolog'Nacional de Ciencia y Tecnolog'ia to JRH by Secretaria de Investigacion y Posgrado-Instituto Politecnico Nacional
- Secretaria de Investigacion y Posgrado-Instituto Politecnico Nacional [20181873, 20180647, 20182010]
- Programa de Apoyo a Proyectos de Investigacion e Innovacion Tecnologica-Direccion General de Asuntos de Personal Academico-Universidad Nacional Autonoma de Mexico [IN214917]
In the present study we determined whether Ustilago maydis accumulates autophagosomes within vacuoles when the cells are exposed to nutritional stress conditions. We investigated whether proteinase B and proteinase A are involved in their degradation. To this effect, wild type and Delta pep4 mutant were incubated in minimal medium lacking a carbon source. It was observed that after incubation in nutrient-deficient media, spherical bodies appeared within the Delta pep4 mutant strains vacuoles. In addition, autophagosomes were accumulated in U. maydis WT cells incubated in the presence of the serine protease inhibitor PMSF and accumulation of large autophagosomes and electrodense structures in the Delta pep4 mutant cell vacuoles took place. These results demonstrate that the homologues of both, the proteinase B and the protease A, are involved in the autophagosomes degradation process in U. maydis.
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