期刊
JOURNAL OF NEUROSCIENCE RESEARCH
卷 98, 期 3, 页码 410-421出版社
WILEY
DOI: 10.1002/jnr.24152
关键词
6-OHDA rat; bioluminescence; chemogenetics; GLucM23; iChloC; optogenetics
资金
- NEI NIH HHS [R21 EY026427] Funding Source: Medline
- NIMH NIH HHS [R21 MH101525] Funding Source: Medline
- NINDS NIH HHS [U01 NS099709] Funding Source: Medline
Previous work has demonstrated that fusion of a luciferase to an opsin, to create a luminescent opsin or luminopsin, provides a genetically encoded means of manipulating neuronal activity via both chemogenetic and optogenetic approaches. Here we have expanded and refined the versatility of luminopsin tools by fusing an alternative luciferase variant with high light emission, Gaussia luciferase mutant GLucM23, to depolarizing and hyperpolarizing channelrhodopsins with increased light sensitivity. The combination of GLucM23 with Volvox channelrhodopsin-1 produced LMO4, while combining GLucM23 with the anion channelrhodopsin iChloC yielded iLMO4. We found efficient activation of these channelrhodopsins in the presence of the luciferase substrate, as indicated by responses measured in both single neurons and in neuronal populations of mice and rats, as well as by changes in male rat behavior during amphetamine-induced rotations. We conclude that these new luminopsins will be useful for bimodal opto- and chemogenetic analyses of brain function.
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