期刊
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
卷 81, 期 3, 页码 169-170出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.diagmicrobio.2014.11.012
关键词
HSV; VZV; Multiplex; Real-time PCR
We compared the performance of 2 multiplex assays (Focus Simplexa and Quidel Lyra) to individual real-time PCR for the detection of herpes simplex virus-1 (HSV-1), HSV-2, and Varicella zoster virus (VZV) from clinical specimens. Results were compared to a consensus standard, defined as the result obtained by at least 2 of the 3 molecular methods. The sensitivity of the Quidel assay ranged from 92.0% for HSV-1 to 97.7% for HSV-2, while the specificity for all targets was 100%. The Focus assay demonstrated 100% sensitivity for all targets, and the percent specificity ranged from 96.8% for HSV-1 to 100% for HSV-2 and VZV. (C) 2015 Elsevier Inc. All rights reserved.
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