期刊
JOURNAL OF NEUROSCIENCE
卷 37, 期 41, 页码 9964-9976出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.1758-17.2017
关键词
AMPA receptor; dendrite; hippocampus; NMDA receptor; sodium imaging; spine
资金
- National Institutes of Health [R21NS085729, R01NS099122]
Dendritic spines are key elements underlying synaptic integration and cellular plasticity, but many features of these important structures are not known or are controversial. We examined these properties using newly developed simultaneous sodium and calcium imaging with single-spine resolution in pyramidal neurons in rat hippocampal slices from either sex. Indicators for both ions were loaded through the somatic patch pipette, which also recorded electrical responses. Fluorescence changes were detected with a high-speed, low-noise CCD camera. Following subthreshold electrical stimulation, postsynaptic sodium entry is almost entirely through AMPA receptors with little contribution from entry through NMDA receptors or voltage-gated sodium channels. Sodium removal from the spine head is through rapid diffusion out to the dendrite through the spine neck with a half-removal time of similar to 16 ms, which suggests the neck has low resistance. Peak [Na+](i) changes during single EPSPs are similar to 5mM. Stronger electrical stimulation evoked small plateau potentials that had significant longer-lasting localized [Na+](i) increases mediated through NMDA receptors.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据