期刊
FEMS YEAST RESEARCH
卷 19, 期 4, 页码 -出版社
OXFORD UNIV PRESS
DOI: 10.1093/femsyr/foz041
关键词
hypoxia; glucose transport; yeast; oxygen sensing; Kluyveromyces lactis
资金
- FEMS Research Grants [FEMS-RG-2016-0053, IT-SIMGBM2012-2]
- EMBO Short Term Fellowship [ASTF358-2012]
- Ateneo Sapienza
- Ministero Affari Esteri e Cooperazione Internazionale, Direzione generale per la Promozione del Sistema Paese [MX14MO08]
Glucose is the preferred nutrient for most living cells and is also a signaling molecule that modulates several cellular processes. Glucose regulates the expression of glucose permease genes in yeasts through signaling pathways dependent on plasma membrane glucose sensors. In the yeast Kluyveromyces lactis, sufficient levels of glucose induction of the low-affinity glucose transporter RAG1 gene also depends on a functional glycolysis, suggesting additional intracellular signaling. We have found that the expression of RAG1 gene is also induced by hypoxia in the presence of glucose, indicating that glucose and oxygen signaling pathways are interconnected. In this study we investigated the molecular mechanisms underlying this crosstalk. By analyzing RAG1 expression in various K. lactis mutants, we found that the bHLH transcriptional activator Sck1 is required for the hypoxic induction of RAG1 gene. The RAG1 promoter region essential for its hypoxic induction was identified by promoter deletion experiments. Taken together, these results show that the RAG1 glucose permease gene is synergistically induced by hypoxia and glucose and highlighted a novel role for the transcriptional activator Sck1 as a key mediator in this mechanism.
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