期刊
ACS APPLIED BIO MATERIALS
卷 2, 期 11, 页码 4812-4825出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsabm.9b00568
关键词
nanorattle; chitosan nanoparticle; stimuli responsive; photothermal therapy; bioimaging; drug delivery
资金
- BioX centre
- Advanced Materials Research Centre (AMRC), Indian Institute of Technology Mandi
- Science and Engineering Research Board (SERB), Government of India [SERB/F/5627/2015-16]
- Department of Biotechnology (DBT), Government of India [BT/PR14749/NNT/28/954/2015]
- IIT Mandi Seed Grant
- MHRD
- DST-INSPIRE [IF160513]
Herein, a stimulus-responsive theranostic nanosystem comprising gold nanorattles (AuNRTs), having a solid octahedron core and thin porous cubic shell, encapsulated within chitosan nanocarriers (CS-AuNRT) has been reported. Due to the plasmonic AuNRTs, CS-AuNRT demonstrated unique features of near infrared (NIR) absorbance and accessible intrinsic electromagnetic hot spots arising due to coupling of inner solid core and outer porous shell. These properties enabled CS-AuNRTs to be used for NIR-responsive drug delivery, photothermal therapy, and surface enhanced Raman scattering (SERS) based bioimaging. Following loading of chemotherapeutic drug doxorubicin (DOX) within AuNRTs along with a phase changing material (PCM), application of NIR irradiation resulted in photothermal melting of the PCM and simultaneous payload release in the surrounding medium. Although being nontoxic themselves, CS-AuNRTs with or without loaded DOX could mount significant cell death in breast cancer cell line (MCF-7) in the presence of NIR light as external stimulus. The oxidative stress generated by DOX-loaded and empty CS-AuNRTs upon NIR irradiation were confirmed by flow-cytometric determination of intracellular reactive oxygen species (ROS). Further, the ROS-led induction of apoptosis in treated MCF-7 cells was established from characteristic nuclear fragmentation, morphological changes and membrane blebbing as observed through confocal fluorescence and scanning electron microscopy. Thus, with NIR responsive chemo-photothermal therapy and SERS based bioimaging, the present nanocarrier system holds potential for cancer theranostics.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据