期刊
MICROBIAL CELL
卷 6, 期 11, 页码 509-523出版社
SHARED SCIENCE PUBLISHERS OG
DOI: 10.15698/mic2019.11.697
关键词
Sulfur dioxide tolerance; Com2 (YER130c); wine preservation; Saccharomyces cerevisiae; stress response
资金
- INNOVINEWINE
- European Regional Development Fund (ERDF) through Norte 2020 [Norte-01-0145-FEDER-000038]
- ERFD through POCI-COMPETE 2020
- FCT-Portuguese Foundation for Science and Technology [PTDC/EXPL/AGR-TEC/1823/2013, PTDC/AGRTEC/3315/2014]
- INTERACT project - Integrated Research in Environment, Agro-Chain and Technology [NORTE-01-0145-FEDER-000017]
- Biosystems and Integrative Sciences Institute (BioISI) by FCT [FCT/UID/Multi/04046/2018]
- iBB-Institute for Bioengineering and Biosciences by FCT [UID/BIO/04565/2019]
- Programa Operacional Regional de Lisboa 2020 [007317, PTDC/AGR-TEC/3315/2014_LISBOA-01-0145-FEDER-016834]
- Fundação para a Ciência e a Tecnologia [PTDC/AGR-TEC/3315/2014] Funding Source: FCT
During vinification Saccharomyces cerevisiae cells are frequently exposed to high concentrations of sulfur dioxide (SO2) that is used to avoid overgrowth of unwanted bacteria or fungi present in the must. Up to now the characterization of the molecular mechanisms by which S. cerevisiae responds and tolerates SO2 was focused on the role of the sulfite efflux pump Ssul and investigation on the involvement of other players has been scarce, especially at a genome-wide level. In this work, we uncovered the essential role of the poorly characterized transcription factor Com2 in tolerance and response of S. cerevisiae to stress induced by SO2 at the enologically relevant pH of 3.5. Transcriptomic analysis revealed that Com2 controls, directly or indirectly, the expression of more than 80% of the genes activated by SO2, a percentage much higher than the one that could be attributed to any other stress responsive transcription factor. Large-scale phenotyping of the yeast haploid mutant collection led to the identification of 50 Com2-targets contributing to the protection against SO2 including all the genes that compose the sulfate reduction pathway (MET3, MET14, MET16, METS, MET10) and the majority of the genes required for biosynthesis of lysine (LYS2, LYS21, LYS20, LYS14, LYS4, LYSS, LYS1 and LYS9) or arginine (ARG5,6, ARG4, ARG2, ARG3, ARG7, ARG8, ORT1 and CPA1). Other uncovered determinants of resistance to SO2 (not under the control of Com2) included genes required for function and assembly of the vacuolar proton pump and enzymes of the antioxidant defense, consistent with the observed cytosolic and mitochondrial accumulation of reactive oxygen species in SO2-stressed yeast cells.
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