期刊
JOURNAL OF MATERIALS CHEMISTRY B
卷 7, 期 43, 页码 6861-6867出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c9tb01641d
关键词
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资金
- National Natural Science Foundation of China [21772188]
- Anhui Provincial Natural Science Foundation [1708085MB33]
- Anhui Province Key Research and Development Program Project [201904d07020012]
Hypochlorous acid (HClO) is a powerful microbicidal agent in the innate immue system; however, abnormal HClO levels can cause tissue damage and many diseases. Thus, it is vitally important to develop facile, rapid and accurate analytical methods for the detection of HClO/ClO(-)in vitro and in vivo. In this work, we have constructed three meso-substituted BODIPY selenides with different hydrocarbyl groups (ethyl for BSe-Et, benzyl for BSe-Bz and phenyl for BSe-Ph) as fluorescent probes for the detection of HClO/ClO-. All three non-fluorescent probes can sense HClO/ClO- to form fluorescent selenoxides by blocking the photo-induced electron transfer process. Their sensing properties display a clear relationship with the structure of the hydrocarbyl. The sensing reactivity is heavily dependent on the electron-donating ability of hydrocarbyls, with the order of the response time as BSe-Et (2 s) < BSe-Bz (5 s) MUCH LESS-THAN BSe-Ph (>100 s). Both BSe-Et and BSe-Bz afford a large fluorescence response and very low detection limits (0.3 nM and 0.8 nM), and BSe-Bz displays a higher selectivity over BSe-Et. Finally, as a representative, BSe-Bz was successfully applied to the detection of exo- and endogenous HClO in living cells, and demonstrated to be a mitochondria-localized fluorescent probe.
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