期刊
JOURNAL OF NANOPARTICLE RESEARCH
卷 19, 期 5, 页码 -出版社
SPRINGER
DOI: 10.1007/s11051-017-3855-1
关键词
Cell cycle; Citrate-coated silver nanoparticles; Nanotoxicity; Raw 264.7. ROS and Viability; Health effects
资金
- European Regional Development Fund (FEDER) through the Competitive Factors Thematic Operational Programme (COMPETE)
- National Funds through the Foundation for Science and Technology (FCT) [CICECO-FCOMP-01-0124FEDER-037271, FCOMP-01-0124-FEDER-021456, FCT PEst-C/CTM/LA0011/2013, FCT PTDC/SAUTOX/120953/2010]
- FCT [SFRH/BD/81792/2011]
- H.O. [SFRH/BPD/111736/2015]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/81792/2011] Funding Source: FCT
Citrate-coated silver nanoparticles (citrate-AgNPs) are among the most commonly used nanomaterials, widely present in industrial and biomedical products. In this study, the cytotoxicity of 30-nm citrate-AgNPs on the macrophage cell line RAW 264.7 was evaluated, using a battery of cytotoxicity endpoints (viability, oxidative stress, and cytostaticity/clastogenicity), at 24 and 48 h of exposure. Citrate-AgNPs decreased cell proliferation and viability only at 75 mu g/mL, suggesting a low sensitivity of RAW cells to lower doses of these AgNPs. After 24 h of exposure, ROS content decreased in cells exposed to 60 mu g/mL AgNPs (IC20 value), corroborating the high tolerance of these cells to citrateAgNPs. However, these cells suffered an impairment of the cell cycle, shown by an increase at the sub-G1 phase. This increase of the sub-G1 population was correlated with an increase of DNA fragmentation, suggesting an increase of apoptosis. Thus, our data are important to understand the effects of low concentrations (IC20) of citrate-AgNPs on in vitro vital macrophage functions.
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