期刊
ADIPOCYTE
卷 8, 期 1, 页码 357-361出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/21623945.2019.1693746
关键词
Acetone precipitation; beige adipocytes; white adipose tissue; UCP1; cleaning method
资金
- National Research Foundation of Korea (NRF) - Ministry of Science, ICT of Korea [NRF-2019R1C1C1007040]
- Ministry of Education [NRF-2019R1I1A1A01060990]
Beige adipocytes, which consume energy mainly in an uncoupling protein 1 (UCP1)-dependent manner, are risen in white adipose tissue (WAT) depots. Since beige adipocyte development is gaining attention as a potential strategy for conquering obesity, worldwide researchers are making efforts to study its biological aspects. However, assessing UCP1 protein levels in beige adipocytes is challenging because of the high level of lipid contaminants in WAT. This study showed that an acetone precipitation method had advantages over conventional methods for eliminating lipid contaminants, achieving clear Western blot bands for WAT proteins, especially UCP1. Our results suggest that the acetone precipitation cleaning method could be useful for the clear analysis and precise evaluation of WAT proteins.
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