期刊
CELL CHEMICAL BIOLOGY
卷 26, 期 11, 页码 1515-+出版社
CELL PRESS
DOI: 10.1016/j.chembiol.2019.09.010
关键词
-
资金
- NIH [GM130183, DK111465, F32GM122357]
Mitochondrial sulfide quinone oxidoreductase (SQR) catalyzes the oxidation of H2S to glutathione persulfide with concomitant reduction of CoQ(10). We report herein that the promiscuous activity of human SQR supported the conversion of CoA to CoA-SSH (CoA-persulfide), a potent inhibitor of butyryl-CoA dehydrogenase, and revealed a molecular link between sulfide and butyrate metabolism, which are known to interact. Three different CoQ(1)-bound crystal structures furnished insights into how diverse substrates access human SQR, and provided snapshots of the reaction coordinate. Unexpectedly, the active site cysteines in SQR are configured in a bridging trisulfide at the start and end of the catalytic cycle, and the presence of sulfane sulfur was confirmed biochemically. Importantly, our study leads to a mechanistic proposal for human SQR in which sulfide addition to the trisulfide cofactor eliminates (201)Cys-SSH, forming an intense charge-transfer complex with flavin adenine dinucleotide, and (379)Cys-SSH, which transfers sulfur to an external acceptor.
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