期刊
JOURNAL OF MICROBIOLOGY
卷 55, 期 10, 页码 767-774出版社
MICROBIOLOGICAL SOCIETY KOREA
DOI: 10.1007/s12275-017-7033-z
关键词
taxonomy; Proteobacteria; Rhodoferax koreense sp nov; sludge
类别
资金
- Next Generation BioGreen 21 Program (SSAC) [PJ0120342017]
- Rural Development Administration, Republic of Korea
Gram-staining-negative, uniflagellated, rod-shaped, designated as DCY110(T), was isolated from sludge located in Gangwon province, Republic of Korea. The phylogenetic tree of 16S rRNA gene sequence showed that the strain DCY110(T) belonged to the genus Rhodoferax with a close similarity to Rhodoferax saidenbachensis DSM 22694(T) (97.7%), Rhodoferax antarcticus DSM 24876(T) (97.5%), Rhodoferax ferrireducens DSM 15236(T) (97.3%), and Rhodoferax fermentans JCM 7819(T) (96.7%). The predominant isoprenoid quinine was ubiquinone (Q-8). DNA G + C content was 62.8 mol%. The major polar lipids were phosphatidylethanolamine and two unidentified phospholipids. The major fatty acids (> 10%) were C-12:0, C-16:0, summed feature 3 (which comprised C-16:1 omega 7c and/or C-16:1 omega 6c). The DNA-DNA relatedness values between the strain DCY110(T) and the closely related relatives used in this study were lower than 70%. Based on the following polyphasic analysis, the strain DCY110(T) is considered as a novel species of the genus Rhodoferax, for which the name Rhodoferax koreense sp. nov. is proposed. The type strain is DCY-110(T) (= KCTC 52288(T) = JCM 31441(T)).
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