期刊
JOURNAL OF MICROBIOLOGICAL METHODS
卷 141, 期 -, 页码 55-59出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2017.07.010
关键词
Leptospira; Loop-mediated isothermal amplification; (LAMP); DNA detection
Leptospirosis, a global zoonotic disease, is often neglected but has a significant impact on human health. Here we reported a new loop mediated isothermal amplification (LAMP) assay targeting lipL32 gene of pathogenic Leptospira spp. Polymerase chain reaction (PCR), nested-PCR and real-time PCR assays were included in this study for the comparison of analytic and diagnostic sensitivity and specificity. LipL32 LAMP we designed enables detection of 10 copies of L. interrogans and has a higher diagnostic sensitivity (91.67%) and specificity (100%) than other PCR-based methods. The high sensitivity, specificity and flexible reaction conditions of the lipL32 LAMP assay makes it feasible for resource -limited countries and on-site application.
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