期刊
DIABETES CARE
卷 38, 期 5, 页码 930-933出版社
AMER DIABETES ASSOC
DOI: 10.2337/dc14-2490
关键词
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资金
- Ministry of Health of the Czech Republic [IGAMZ 11465-5]
- Academy of Finland
- Project for the Conceptual Development [00064203]
- EU [CZ.2.16/3.1.00/24022OPPK]
- JDRF
- Academy of Finland [129448, 255770, 132362]
- Centre of Excellence in Molecular Systems Immunology and Physiology Research [20122017, 250114]
- Sigrid Juselius Foundation
- Competitive Research Funding of University Hospital in Oulu, Tampere
- Competitive Research Funding of University Hospital in Oulu, Turku
- European Union [261441]
- Academy of Finland (AKA) [255770, 129448, 132362, 255770, 132362, 129448] Funding Source: Academy of Finland (AKA)
OBJECTIVEThis study used next-generation sequencing (NGS) technologies to characterize the gut virome at the onset of islet autoimmunity.RESEARCH DESIGN AND METHODSWe conducted a case-control study nested within the Finnish Diabetes Prediction and Prevention (DIPP) cohort. The stool virome in 19 case children, who turned islet autoantibody positive before the age of 2 years and later developed clinical type 1 diabetes, and 19 tightly matched control subjects was analyzed using NGS performed from stool samples collected 3, 6, and 9 months before the onset of islet autoimmunity. Human virus findings were verified using real-time PCR.RESULTSOne or more human viruses were present in 10.4% and bacteriophages were in 54% of the samples. The virome composition showed no association with islet autoimmunity. NGS was less sensitive and specific than real-time PCR.CONCLUSIONSThe present data suggest no dramatic changes in the gut virome shortly before the emergence of islet autoimmunity and emphasize the need of verification of mass sequencing results when viral exposure is assessed in association studies.
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