期刊
NANOSCALE
卷 11, 期 44, 页码 21119-21127出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c9nr06256d
关键词
-
类别
资金
- Ministry of Science and Technology of Taiwan
- Genomics Research Center, Academia Sinica, Taiwan [MOST-104-2113-M-001-015-MY3, MOST 105-0210-01-13-01, MOST 105-2113-M-005-016-MY2, MOST 107-2113-M-005-014-, MOST 108-2113-M-005-004-, MOST 107-2119-M-001-039-, AS-105-TP-A04, AS-iMATE-107-51]
- Taiwan Ministry of Health and Welfare, under the welfare surcharge of tobacco products [CMRPG3C1141]
An immunomagnetic nano-net was designed and synthesized for specifically capturing rare cells of interest from mixtures. The nano-net, Ab@Lipo-MNP-GO, consists of conjugated antibody molecules on a lipid coated magnetic nanoparticle-graphene oxide sheet complex. The magnetism, chemical composition, and the morphology of the construct and its precursors were characterized by SQUID, FTIR, TGA, DLS and SEM, to confirm the feasibility of the synthetic steps and the resulting properties suitable for solution phase immuno-recognition for cell capture. When applied to capturing circulating tumor cells (CTCs) in oral, colon and lung cancer clinical patients' blood samples, the nano-net construct exhibited far superior ability whereas conventional immunomagnetic beads in some cases were unable to capture any CTCs, even by increasing the bead concentration. Confocal images showed that the nano-net wrapped around the CTCs while the immunomagnetic beads attached them with point contacts. A stable, patch-like multivalent matrix nano-net was demonstrated to tackle the shortcomings of single point contact of immunomagnetic beads to the target cell. This strategy is universal for any cell separation in complex fluids.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据