期刊
CURRENT DEVELOPMENTS IN NUTRITION
卷 3, 期 12, 页码 -出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/cdn/nzz122
关键词
CXCL9; exosomes; inflammatory bowel disease; Mdr1a(-/-) mice; microRNA
资金
- National Institute of Food and Agriculture [2015-67017-23181, 2016-67001-25301]
- NIH [1P20GM104320]
- USDA [W4002]
- National Institute for General Medical Science (NIGMS) grants [INBRE-P20GM103427-14, COBRE-1P30GM110768-01]
- Fred & Pamela Buffett Cancer Center Support Grant [P30CA036727]
- NIH (NIGMS) COBRE IDeA award [1P20GM104320]
Background: Exosomes transfer regulatory microRNAs (miRs) from donor cells to recipient cells. Exosomes and miRs originate from both endogenous synthesis and dietary sources such as milk. miR-200a-3p is a negative regulator of the proinflammatory chemokine (C-X-C motif) ligand 9 (CXCL9). Male Mdr1a(-/-) mice spontaneously develop clinical signs of inflammatory bowel disease (IBD). Objectives: We assessed whether dietary depletion of exosomes and miRs alters the severity of IBD in Mdr1a(-/-) mice owing to aberrant regulation of proinflammatory cytokines. Methods: Starting at 5 wk of age, 16 male Mdr1a(-/-) mice were fed either milk exosome- and RNA-sufficient (ERS) or milk exosome- and RNA-depleted (ERD) diets. The ERD diet is characterized by a near-complete depletion of miRs and a 60% loss of exosome bioavailability compared with ERS. Mice were killed when their weight loss exceeded 15% of peak body weight. Severity of IBD was assessed by histopathological evaluation of cecum. Serum cytokine and chemokine concentrations and mRNA and miR tissue expression were analyzed by multiplex ELISAs, RNA-sequencing analysis, and qRT-PCR, respectively. Results: Stromal collapse, gland hyperplasia, and additive microscopic disease scores were (mean +/- SD) 56.7% +/- 23.3%, 23.5% +/- 11.8%, and 29.6% +/- 8.2% lower, respectively, in ceca of ERS mice than of ERD mice (P < 0.05). The serum concentration of CXCL9 was 35.0% +/- 31.0% lower in ERS mice than in ERD mice (P < 0.05). Eighty-seven mRNAs were differentially expressed in the ceca from ERS and ERD mice; 16 of these mRNAs are implicated in immune function. The concentrations of 4 and 1 out of 5 miRs assessed (including miR-200a-3p) were <= 63% lower in livers and ceca, respectively, from ERD mice than from ERS mice. Conclusions: Milk exosome and miR depletion exacerbates cecal inflammation in Mdr1a(-/-) mice.
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