期刊
GLYCOBIOLOGY
卷 29, 期 12, 页码 817-821出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/glycob/cwz061
关键词
biomarker; dried blood spots; high-throughput; IgG glycome; ultra-performance liquid chromatography
资金
- European Structural and Investment Funds IRI [KK.01.2.1. 01.0003]
- Croatian Centre of Research Excellence in Personalized Healthcare [KK.01.1.1.01.0010]
- European Commission through Horizon 2020 programme, Marie-Sklodowska Curie European Training Network [676421]
- Ministry of Science and Education of the Republic of Croatia [309-0061194-2023]
- European Commission through European Structural and Investment funds 2014-2020
- European Commission through Horizon 2020
- Ministry of Science, Education and Sports of the Republic of Croatia
Immunoglobulin G (IgG) glycans are emerging as a new putative biomarker for biological age and different diseases, requiring a robust workflow for IgG glycome analysis, ideally beginning with a simple and undemanding sampling procedure. Here, we report the first comprehensive study on total N-glycans of IgG isolated from dried blood spots (DBSs), which was performed in a high-throughput mode. We compared the IgG N-glycan profiles originating from DBS with those originating from plasma, compared different media for DBS collection, evaluated analytical variation and assessed IgG N-glycan profile stability for different storage conditions. In conclusion, we show that DBSs are a good and stable source material for a robust IgG N-glycan analysis by ultra-performance liquid chromatography, suitable for blood sampling in conditions where no trained personnel and necessary laboratory equipment are available.
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