期刊
JOURNAL OF LIPID RESEARCH
卷 58, 期 9, 页码 1924-1931出版社
ELSEVIER
DOI: 10.1194/jlr.D076661
关键词
lipid peroxidation; fatty acid/oxidation; oxidized lipids; quantitation; mass spectrometry; derivatization; 2,4-dinitrophenylhydrazine; ultra-high-performance liquid chromatography-high-resolution mass spectrometry
资金
- Swiss National Science Foundation [PP00P3_139011]
- Swiss National Science Foundation (SNF) [PP00P3_139011] Funding Source: Swiss National Science Foundation (SNF)
Quantification of malondialdehyde (MDA) as a marker of lipid peroxidation is relevant for many research fields. We describe a new sensitive and selective method to measure free and total plasmatic MDA using derivatization with 2,4-dinitrophenylhydrazine (DNPH) and ultra-HPLChigh- resolution MS. Free and total MDA were extracted from minute sample amounts (10 mu l) using acidic precipitation and alkaline hydrolysis followed by acidic precipitation, respectively. Derivatization was completed within 10 min at room temperature, and the excess DNPH discarded by liquid-liquid extraction. Quantification was achieved by internal standardization using dideuterated MDA as internal standard. The method's lowest limit of quantification was 100 nM and linearity spanned greater than three orders of magnitude. Intra-and inter-day precisions for total MDA were 2.9% and 3.0%, respectively, and those for free MDA were 12.8% and 24.9%, respectively. Accuracy was 101% and 107% at low and high concentrations, respectively. In human plasma, free MDA levels were 120 nM (SD 36.26) and total MDA levels were 6.7. M (SD 0.46). In addition, we show the applicability of this method to measure MDA plasma levels from a variety of animal species, making it invaluable to scientists in various fields.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据