期刊
JOURNAL OF LIPID RESEARCH
卷 58, 期 6, 页码 1055-1066出版社
ELSEVIER
DOI: 10.1194/jlr.M071506
关键词
CHRNA7; CHRNA9; CHRNA10; dipalmitoylphosphatidylcholine; inflammasome; monocyte; purinergic receptor P2X 7; phosphatidylcholine
资金
- German Centre for Lung Research (DZL) grant
- German Research Foundation [GR 1094/7-1]
- National Institutes of Health [P01-GM48677, R01-GM103801]
Interleukin (IL)-1 beta is a potent pro-inflammatory cytokine of innate immunity involved in host defense. High systemic IL-1 beta levels, however, cause life-threatening inflammatory diseases, including systemic inflammatory response syndrome. In response to various danger signals, the proform of IL-1 beta is synthesized and stays in the cytoplasm unless a second signal, such as extracellular ATP, activates the inflammasome, which enables processing and release of mature IL-1 beta. As pulmonary surfactant is known for its anti-inflammatory properties, we hypothesize that surfactant inhibits ATP-induced release of IL-1 beta. Lipopolysaccharide-primed monocytic U937 cells were stimulated with an ATP analog in the presence of natural or synthetic surfactant composed of recombinant surfactant protein (rSP)-C, palmitoylphosphatidylglycerol, and dipalmitoylphosphatidylcholine (DPPC). Both surfactant preparations dose-dependently inhibited IL-1 beta release from U937 cells. DPPC was the active constituent of surfactant, whereas rSP-C and palmitoylphosphatidylglycerol were inactive. DPPC was also effective in primary mononuclear leukocytes isolated from human blood. Experiments with nicotinic antagonists, siRNA technology, and patch-clamp experiments suggested that stimulation of nicotinic acetylcholine receptors (nAChRs) containing subunit alpha 9 results in a complete inhibition of the ion channel function of ATP receptor, P2X7. In conclusion, the surfactant constituent, DPPC, efficiently inhibits ATP-induced inflammasome activation and maturation of IL-1 beta in human monocytes by a mechanism involving nAChRs.
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