期刊
JOURNAL OF LIPID RESEARCH
卷 58, 期 11, 页码 2147-2161出版社
ELSEVIER
DOI: 10.1194/jlr.M079764
关键词
Plin2; triacylglycerol; lipid droplet; lipolysis and fatty acid metabolism; fatty acid/metabolism; insulin signaling; muscle
资金
- Medical Faculty at the University of Oslo
- Johan Throne-Holst Foundation
- Intramural Research Programs of the National Institutes of Health (NIH)
- National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
- Novo Nordisk Foundation
- Aktieselskabet Freia Chocolade Fabrik's Medical Foundation
- Anders Jahre's Foundation
Lipid droplet (LD) coating proteins are essential for the formation and stability of intracellular LDs. Plin2 is an abundant LD coating protein in skeletal muscle, but its importance for muscle function is unclear. We show that myotubes established from Plin2(-/-) mice contain reduced content of LDs and accumulate less oleic acid (OA) in triacylglycerol (TAG) due to elevated LD hydrolysis in comparison with Plin2(+/+) myotubes. The reduced ability to store TAG in LDs in Plin2(-/-) myotubes is accompanied by a shift in energy metabolism. Plin2(-/-) myotubes are characterized by increased oxidation of OA, lower glycogen synthesis, and reduced glucose oxidation in comparison with Plin2(+/+) myotubes, perhaps reflecting competition between FAs and glucose as part of the Randle cycle. In accord with these metabolic changes, Plin2(-/-) myotubes have elevated expression of Ppara and Ppargc1a, transcription factors that stimulate expression of genes important for FA oxidation, whereas genes involved in glucose uptake and oxidation are suppressed. Loss of Plin2 had no impact on insulin-stimulated Akt phosphorylation. Our results suggest that Plin2 is essential for protecting the pool of skeletal muscle LDs to avoid an uncontrolled hydrolysis of stored TAG and to balance skeletal muscle energy metabolism.
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