期刊
ARHIV ZA HIGIJENU RADA I TOKSIKOLOGIJU-ARCHIVES OF INDUSTRIAL HYGIENE AND TOXICOLOGY
卷 70, 期 4, 页码 303-309出版社
INST MEDICAL RESEARCH & OCCUPATIONAL HEALTH
DOI: 10.2478/aiht-2019-70-3322
关键词
black thorn; cytotoxicity; Hep G2; kenacid blue; neutral red; ROS
资金
- Croatian Science Foundation [3035]
Prunus spinosa L. (blackthorn) is used in traditional medicine as a remedy for various diseases. To establish its anticancer properties, we exposed human liver cancer cells (Hep G2) to a range of blackthorn flower extract concentrations (10-200 mu g/mL) and determined cytotoxic activity with the neutral red and kenacid blue methods after 24. 48. and 72 h of incubation. Statistically significant inhibitory effects on Hep G2 cellular proliferation were observed at concentrations above 50 mu g/mL (p<0.001-0.05). Cell viability was lower when determined with neutral red than kenacid blue method. In addition, we evaluated antioxidant/prooxidant effects of the blackthorn flower extract by measuring reactive oxygen species (ROS), and the results confirmed its prooxidant behaviour within the applied concentration range. Flow cytometry determined primarily necrotic and apoptotic cell death, which provides additional evidence of its cytotoxic effect on liver carcinoma.
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