期刊
ISCIENCE
卷 22, 期 -, 页码 133-+出版社
CELL PRESS
DOI: 10.1016/j.isci.2019.11.011
关键词
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资金
- Brain Research Center from The Featured Areas Research Center Program within the Ministry of Education (MOE) in Taiwan
- Ministry of Science and Technology (MOST) in Taiwan
- MOST [MOST-108-2321-B-002-004, MOST-105-2628-M-002-010-MY4, MOST-107-2221-E-007-036-MY2, MOST106-2221-E-007-059, MOST104-2218-E-007-022-MY2]
- Foundation for the Advancement of Outstanding Scholarship during 2015-2018
All-optical physiology (AOP) manipulates and reports neuronal activities with light, allowing for interrogation of neuronal functional connections with high spatiotemporal resolution. However, contemporary high- speed AOP platforms are limited to single-depth or discrete multi-plane recordings that are not suitable for studying functional connections among densely packed small neurons, such as neurons in Drosophila brains. Here, we constructed a 3D AOP platform by incorporating single-photon point stimulation and two- photon high-speed volumetric recordings with a tunable acoustic gradient-index (TAG) lens. We demonstrated the platform effectiveness by studying the anterior visual pathway (AVP) of Drosophila. We achieved functional observation of spatiotemporal coding and the strengths of calcium-sensitive connections between anterior optic tubercle (AOTU) sub-compartments and >70 tightly assembled 2-mu m bulb (BU) microglomeruli in 3D coordinates with a single trial. Our work aids the establishment of in vivo 3D functional connectomes in neuron-dense brain areas.
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