期刊
NEOTROPICAL BIODIVERSITY
卷 5, 期 1, 页码 69-74出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/23766808.2019.1706387
关键词
DNA extraction; low-cost protocol; DNA precipitation; DNA from blood; DNA from swabs
资金
- National Geographic Society [NGS-421C-18]
- Galapagos Science Center [POA-GSC-2019]
- Universidad San Francisco de Quito [HUBI 12266]
- [CONICYT PIA APOYO CCTE AFB170008]
- [FONDECYT 3170211]
Laboratories that perform PCR on a routine basis need to count on reliable DNA isolation methods. In locations where supply of DNA extraction kits depends on importation, having an in-house protocol is desirable. This is also important for laboratories limited by budget constraints. We present a low-cost DNA isolation protocol that incorporates well-known techniques, but that we have adapted to various animal tissues. We tested this protocol on animal blood and muscle, and on cell suspension from skin swabs. The results were comparable, in terms of amount and quality of DNA, to those obtained with two other commercially available methods. DNA retrieved with this protocol has been successfully employed for Sanger sequencing of gene PCR products from animal tissues and blood, as well as for PCR-based diagnosis of chytrid fungus in amphibians and blood parasites in birds.
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