期刊
NEW JOURNAL OF CHEMISTRY
卷 43, 期 47, 页码 18517-18524出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c9nj04426d
关键词
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资金
- National Natural Science Foundation of China [21708027]
- Natural Science Foundation of Capital Medical University in China [2015ZR16]
A novel luminescent chemosensor based on an alpha,beta-unsaturated ketone conjugated Ru(bpy)(3)(2+) derivative (Ru-E) has been designed and synthesized for the highly selective and sensitive detection of cysteine in 100% aqueous solutions. Owing to the conjugate 1,4-addition of cysteine to alpha,beta-unsaturated ketones, the non-luminescent chemosensor Ru-E could specifically react with cysteine to form the corresponding product, Ru-Cys, accompanied by remarkable luminescence enhancement at 620 nm. Job's plot and mass spectrometry analysis indicate a 1:2 binding stoichiometry between Ru-E and Cys. The sensing mechanism is confirmed by NMR and mass and emission spectrometry. Moreover, Ru-E exhibited very low cytotoxicity and was successfully applied to image endogenous thiols both in living bone mesenchymal stem cells (BMSCs) and zebrafish. In addition, Ru-E showed biological application for monitoring thiols in newborn-calf serum. The results of this work demonstrate that Ru-E would be a useful tool for physiological and pathological studies involving biothiols.
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