期刊
JOURNAL OF INORGANIC BIOCHEMISTRY
卷 177, 期 -, 页码 138-142出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jinorgbio.2017.08.036
关键词
Metal-organic framework; Fluorescence sensing; MiRNAs; Gastric cancer
资金
- Guangdong Provincial Department of Science and Technology of China [2015A010105016]
- Guangdong Provincial Natural Science Foundation of China [2015A030313284]
- State Key Laboratory of Quality Research in Chinese Medicine (Macau University of Science and Technology) - Macao Science and Technology Development Fund [MUST-SKL-2016-04]
- Macau Special Administrative Region
- Special Funds for the Cultivation of Guangdong College Students' Scientific and Technological Innovation. (Climbing Program Special Funds)
We report herein five sensing platforms for the detection of five gastric cancer associated microRNAs (miRNAs). The sensing platforms are hybrids formed from a water-stable metal organic framework (MOF) {[Cu (dcbb)(2)(H2O)(2)]center dot 10H(2)O}(n) (1, H(2)dcbbBr = 1-(3,5-dicarboxybenzy1)-4,4'-bipyridinium bromide), respectively with five carboxyfluorescein (FAM) labeled probe single-stranded DNA (probe ss-DNA, denoted as P-DNA). Within the hybrid, MOF 1 tightly interacts with the P-DNA through electrostatic and/or n-stacking interactions and results in fluorescence quenching of FAM via a photo-induced electron transfer (PET) process. In the presence of the complementary target miRNAs miR-185, miR-20a, miR-92b, miR-25 and miR-210, which are expressed abnormally in the plasma of gastric carcinoma patients, P-DNA is released from the surface of MOF 1 ascribed to the stronger base pair matching, leading to the FAM fluorescence recovery. Each P-DNA@1 system is effective and reliable for the detection of its complementary target miRNA with the detection limits from 91 to 559 pM, and is not interfered by other four miRNA sequences.
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