期刊
JOURNAL OF INORGANIC BIOCHEMISTRY
卷 174, 期 -, 页码 45-54出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jinorgbio.2017.05.015
关键词
Vanadium; DNA crosslinking; Mitochondria targeting; Terpyridine derivative; Photocytotoxicity; Crystal structure
资金
- Department of Science and Technology, Government of India [SR/S5/MBD-02/2007, EMR/2015/000742]
- J.C. Bose fellowship
- CSIR
- University Grants Commission (UGC), New Delhi
- DBT-IISc
- DST-FIST
- IISc Bangalore
Oxovanadium(IV) complexes [VO(L-1/L-2)Cl-2](n+) (1,2) of (anthracenyl)terpyridine (An-tpy as L-1 in 1, n = 0) and triphenylphosphonium-appended (anthracenyl)terpyridine (An-tpy-TPP+ as L-2 in 2, n = 1) were synthesized, characterized and their DNA crosslinking ability, photocytotoxicity in visible light and cellular localization in cancer cells studied. The bromide derivative of 2, viz. [VO(An-tpy-TPP)Br-2]Br (3) is structurally characterized. The structure showed trans disposition of two halides in the coordination sphere and the TPP+ unit is a pendant to the terpyridyl ligand. The DNA melting and comet assay studies on the complexes suggest the formation of DNA crosslinks. Complexes 1 and 2 displayed similar to 10 fold increase in cytotoxicity on exposure to visible light (400-700 nm) when compared to those in dark in HeLa and MCF-7 cells. FACScan (Fluorescence Associated Cell Sorter Scan) analysis showed cellular apoptosis when treated with the complex in visible light in comparison to their dark controls. Fluorescence microscopic studies using complex 2 revealed its mitochondrial localization within the cancer cells.
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