4.7 Article

Gold Nanostars for the Detection of Foodborne Pathogens via Surface-Enhanced Raman Scattering Combined with Microfluidics

期刊

ACS APPLIED NANO MATERIALS
卷 2, 期 10, 页码 6081-+

出版社

AMER CHEMICAL SOC
DOI: 10.1021/acsanm.9b01223

关键词

gold nanostars; microfluidics; optofluidics; SERS-on-a-chip; foodborne pathogens; Listeria monocytogenes

资金

  1. EU Framework Programme for Research and Innovation H2020 COFUND [713640]
  2. Nano technology Based Functional Solutions [NORTE-01-0145-FEDER-000019]
  3. Norte Portugal Regional Operational Programme (NORTE2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund
  4. U.S. Department of Agriculture, Agricultural Research Service [59-8072-6-001]

向作者/读者索取更多资源

Herein, we demonstrated the potential of surface enhanced Raman scattering (SERS) spectroscopy combined with microfluidics for the detection and discrimination of foodborne pathogens. SERS-tagged gold nanostars were functionalized with a monoclonal antibody specific for Listeria monocytogenes. In the presence of L. monocytogenes, a SERS signal corresponding to the SERS tag paired to the antibody was detected in real time and in continuous flow, enabling the discrimination of L. monocytogenes and Listeria innocua in just 100 s. To the best of our knowledge, this is the first time that SERS tags have been used for the in-flow detection of living organisms.

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