期刊
JOURNAL OF INFECTIOUS DISEASES
卷 215, 期 7, 页码 1049-1058出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/infdis/jix070
关键词
respiratory syncytial virus; viral infection; infants; bronchiolitis; CD4(+) T cells; interleukin 2; interferon gamma; markers of disease severity
资金
- Fondo Nacional para la Investigacion Cientifica y Tecnologica [PIDC 0010-2015, PMO BID PICT 2014-1578]
- CONICET (PIP) [115]
- Universidad de Buenos Aires [20020130100446BA]
Background. Although human airway epithelial cells are the main target of respiratory syncytial virus (RSV), it also infects immune cells, such as macrophages and B cells. Whether T cells are permissive to RSV infection is unknown. We sought to analyze the permissiveness of CD4(+) T cells to RSV infection. Methods. CD4(+) and CD8(+) T cells from cord blood, healthy young children, and adults were challenged by RSV or cocultured with infected HEp-2 cells. Infection, phenotype, and cytokine production by T cells were analyzed by flow cytometry or enzyme-linked immunosorbent assay. Expression of RSV antigens by circulating CD4(+) T cells from infected children was analyzed by flow cytometry, and disease severity was defined by standard criteria. Results. CD4(+) and CD8(+) T cells were productively infected by RSV. Infection decreased interleukin 2 and interferon. production as well as the expression of CD25 and Ki-67 by activated CD4(+) T cells. Respiratory syncytial virus antigens were detected in circulating CD4(+) and CD8(+) T cells during severe RSV infection of young children. Interestingly, the frequency of CD4(+) RSV+ T cells positively correlated with disease severity. Conclusions. Respiratory syncytial virus infects CD4(+) and CD8(+) T cells and compromises T-cell function. The frequency of circulating CD4(+) RSV+ T cells might represent a novel marker of severe infection.
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